This advanced practical course introduces compound microscopy and slide preparation techniques used for small Diptera, Hymenoptera, genitalia dissections and diagnostic morphological work.

Advanced Microscopy

• Compound microscope setup
• Köhler illumination (introductory level)
• Magnification calibration
• Measuring structures
• Understanding diagnostic morphology

Dissection & Clearing

• Softening specimens
• Potassium hydroxide (KOH) overview
• Clearing tissues
• Safe handling protocols

Slide Mounting Techniques

• Temporary slide mounts
• Permanent slide mounting principles
• Mounting media overview
• Cover slip application
• Avoiding air bubbles
• Labelling slides correctly

When Slide Work Is Necessary

• Recognising diagnostic structures
• Limitations of field ID
• Responsible identification claims
• Knowing when to consult specialists